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Figure 2


Fig. 2. Cohesin SMCs localize along chromosome cores of meiotic chromosomes. A chromosome spread slide was treated with buffer alone (top panels) or with DNase I (bottom panels) and immunostained for SMC1/3 (green), ORD (orange), histone (blue) and DNA (white). Although histone and DAPI staining is lost when chromosome loops are digested with DNase I, SMC1/3 and ORD signals remain visible along chromosome cores. For each fluorophore, identical exposure times were used to capture cells treated with DNase and not treated with DNase. Bar, 2 µm.





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