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Figure 4


Fig. 4. ORD is required for centromeric localization of cohesin SMCs. (Top panels) Wild-type chromosome spread preparation immunostained for SMC1/3 (green), CID (orange) and C(3)G (magenta). Note that the two bright SMC1/3 patches overlap with the CID signal at the centromeres and thread-like SMC and C(3)G signals are visible along chromosome arms. Insets contain an enlarged view of the pericentric region (arrow) and show that enrichment of SMC1/3 staining extends beyond the CID signal. (Bottom panels) Single nucleus from a ordnull chromosome spread preparation immunostained for SMC1/3 (green), CID (orange) and C(3)G (magenta). Distinct gaps in the thread-like SMC signal correspond to CID foci, indicating that – in the absence of ORD – cohesin SMCs fail to accumulate at meiotic centromeres. Bar, 2 µm.





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