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Figure 5


Fig. 5. Cohesin SMCs exhibit a distinct localization pattern at the centromeres of nurse cell chromosomes that is not dependent on ORD. Vitellarial stage-3 egg chambers (see schematic on right) from a whole-mount ord+ ovariole (top panels) and an ordnull ovariole (bottom panels) were immunostained for SMC1/3 (green) and DNA (white). Note that SMC staining in both wild-type and mutant nurse cell nuclei coincides with bright DAPI-stained regions (solid arrows). Insets correspond to a magnified view of each region indicated by a solid arrow. Note the SMC1/3 finger-like projections. Open arrows indicate SMC1/3 staining in the oocyte nucleus. All images are projections of deconvolved Z-series. The insets include only a subset of sections shown for the entire egg chamber. Bar, 10 µm. The schematic on the right indicates the stage of the cysts shown.





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