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Figure 8


Fig. 8. Fragmentation of chromosome cores is detectable before C(3)G localization defects become apparent in ordnull germaria. (A) Graph comparing SMC1 and SMC3 localization defects in ordnull germaria (see Tables 1 and 2) with those observed for C(3)G (Webber et al., 2004). (B) Individual nuclei from whole-mount preparations of ordnull germaria were simultaneously immunostained for SMC1/3 (green) and C(3)G (magenta). In early region 2A, cohesin SMC and C(3)G signals coincide extensively. Although long stretches of thread-like C(3)G staining are still visible in late region 2A cysts, SMC1/3 staining is severely fragmented. When fragmentation of C(3)G becomes apparent, SMC staining is limited to very short fragments and puncta. Images are projections of deconvolved Z-series. SMC images from different stages were captured and processed identically as were the C(3)G images from late region 2A and region 2B. Bar, 2 µm.





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