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Figure 3


Fig. 3. Inhibition of Ras but not Rac reduces PtdIns(3,4,5)P3 production at the leading edge of lamellipodia. (A,B) MTLn3 cells were transfected with control or Rac1 siRNA. (A) Representative images of control siRNA or Rac1 siRNA-treated cells stimulated with EGF for 3 minutes and stained with anti-PtdIns(3,4,5)P3 antibody are shown. (B) Quantification of leading-edge PtdIns(3,4,5)P3 in siRNA-treated cells. The data are mean ± s.e.m. from four experiments. Bar, 10 µm in all panels. (C,D) MTLn3 cells were transfected with control siRNA or pools of four siRNA duplexes specific for H-Ras, K-Ras or N-Ras. (C) Representative images of control siRNA or K-Ras siRNA-treated cells stimulated with EGF for 0, 1 or 3 minutes and stained with anti-PtdIns(3,4,5)P3 antibody are shown. (D) Quantification of leading-edge PtdIns(3,4,5)P3 in siRNA-treated cells. The data are mean ± s.e.m. from 40 cells per condition, and are representative of two independent experiments.





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