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Figure 4


Fig. 4. Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion. (A) MTLn3 cells were transfected with control or Rac1 siRNA. Time-lapse images of EGF-stimulated cells were recorded (20 seconds per frame) with a 20x 0.4 NA objective, and surface areas were measured using NIH Image. The data show the mean ± s.e.m. from 41 cells per time point. (B) Data from A were normalized to the initial area of each cell. (C) MTLn3 cells were transfected with control siRNA or isoform-specific siRNA targeting H-Ras, K-Ras, N-Ras or both N-Ras and K-Ras. The surface area of control siRNA or Ras siRNA-treated cells was measured after various times of EGF stimulation. Cell areas were normalized to the initial area of each cell. Data show the mean ± s.e.m. from 9-14 cells per time point. (D) Pooled data from two experiments showing maximal EGF-stimulated protrusion in cells treated with control or anti-Ras siRNA. Data are the mean ± s.d.





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