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Figure 1


Fig. 1. Stimulated exocytosis detected by annexin V binding. (A,B) Substantial differences are observed for exocytosis stimulated by patterned bilayers (A) with and (B) without lipid ligand DNP-cap-DPPE (`Ag'). (C,D) The Alexa Fluor-488-annexin V label (green) is localized to cells on patterned regions (red) compared with cells (reflected light overlay) off patterns when only half of the chip is covered by haptenated lipids (C), but it is not localized to the defined regions of stimulus at the subcellular level (D). Cells were incubated with the patterns for 30 minutes at 37°C. Bars, 100 µm (A-C), 20 µm (D).





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