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Figure 4


Fig. 4. Recycling endosomes labeled by Alexa Fluor-488 CTB-GM1 are targeted locally to the site of initial stimulus. (A) Confocal fluorescence images illustrate the intracellular pool of CTB-GM1 (equatorial view) and the enrichment of CTB-GM1 over the patterned bilayer (interfacial view). (B,C) Differential mobility of (B) Lyn-EGFP and (C) CTB-GM1 in patterns measured by FPR. (D,E) Slow mobility of surface CTB-GM1 measured by FPR in unstimulated cell (D) with or (E) without the internal perinuclear pool fluorescently labeled to assess contribution of outward trafficking to the fluorescence recovery on the plasma membrane. Bars, 25 µm (A), 10 µm (B-E).





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