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Figure 8


Fig. 8. Internalization of cell-surface tTG requires the endocytic receptor LRP1. LRP1 deficiency inhibits internalization of cell-surface tTG. Wild-type (CHO wt) and LRP1-deficient (CHO 13-5-1) CHO cells (A) or normal (MEF) and Lrp1–/– (PEA-13) mouse embryonic fibroblasts expressing exogenous tTG (B) were surface-biotinylated with sulfo-NHS-SS-biotin and subjected to internalization assays. (A,B) Biotinylated proteins were isolated and tTG was detected by SDS-PAGE and immunoblotting in the fraction of proteins internalized from the cell surface. Proteolytic fragments of tTG are marked with asterisks. Graphs on the right show the levels of internalized tTG for each cell type at different time points of internalization. The values presented are expressed as percentages of those obtained for cell-surface tTG before internalization. The results are representative of three independent experiments performed for each cell type (means ± s.d.).





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