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Figure 4


Fig. 4. Par-6 represses whereas Cdc42 partially relieves aPKC kinase activity. (A) Kinase activity of aPKC, Par-6–aPKC, and Cdc42–Par-6–aPKC complexes. The high intrinsic kinase activity of aPKC, expressed and purified from HEK 293 cells, is efficiently repressed by addition of full-length Par-6. Par-6 has no effect on PKC{alpha} (right panel). Cdc42 partially restores aPKC activity. The signal is from a rhodamine-labeled peptide corresponding to a PKC consensus substrate (sequence shown on left). (B) aPKC fractionates predominantly with Par-6. Fractions of Drosophila embryonic lysate from stages 8 to 14 embryos from a calibrated gel filtration column are shown western blotted with both anti-aPKC and anti-Par-6 antibodies. Very little aPKC fractionates at its native molecular mass (~80 kD) but, instead, co-fractionates with Par-6. (C) Pathway for regulation of apical complex activity in neuroblasts.





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