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Fig. 7. Effect of nocodazole treatment on Golgi dispersion in poliovirus-infected cells. The localization of two different Golgi markers, GM130 and β-COP, were monitored by confocal immunofluorescence microscopy. (A-L) HeLa cells were pretreated with 10 µg/ml nocodazole (+Noc) or with medium without nocodazole (–Noc) for 1.5 hours Cells were then mock-infected (uninfected) or infected with poliovirus (MOI=10 PFU/ml) in the presence or absence of 10 µg/ml nocodazole. Cells were fixed at 3.5 hours post infection. Cells grown on cover slips were processed for indirect immunofluorescence and confocal microscopy as shown; the other cells in the dish were processed for conventional EM (see Fig. 8). The Golgi complex was stained with a murine monoclonal antibody directed against GM130 (red; A-D) and an affinity-purified polyconal rabbit antibody against β-COP (green, E-H) that detects COPI present on Golgi, ERGIC and COPI vesicular structures.
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