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Figure 1


Fig. 1. (A,B) PML is targeted to the nucleolus upon cellular stresses. Confocal images of MRC5 human fibroblasts treated with 35 J/m2 UVC radiation (1 hour), 2 µg/ml doxorubicin (Doxo) for 24 hours, 5 nM actinomycin D (ActD) for 16 hours, 10 µM MG132 for 16 hours, or 10 Gy ionizing radiation {gamma} (IR-{gamma}) (1 hour). Immunofluorescence was performed using antibodies against PML, B23, fibrillarin or UBF, as indicated. (B) Summary of the localization patterns observed in Pml–/– MEFs expressing PML isoforms -I, -II, -III, -IV or -V, and treated as indicated. NBs, nuclear bodies. (C) PML-I targets nucleolar caps upon doxorubicin treatment. MRC5 cells were transfected with PML-I specific or control siRNA and treated with doxorubicin for 24 hours. Immunofluorescence was then performed using Pan-PML or PML-I-specific sera, as indicated. Nuclei were stained with DAPI.





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