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Figure 1


Fig. 1. Cloning and mutation of H. pomatia synapsin. (A) Sequence alignment of the newly cloned H. pomatia synapsin (helSyn) and the closely related A. californica synapsin (apSyn) isoform 11.1. Asterisks indicate amino acid identities. The conserved structural domains A, C and E are highlighted. (B) Sequence alignment of the domain A of vertebrate and invertebrate synapsin isoforms. The highly conserved PKA/CaMKI/IV phosphorylation site (site 1) corresponding to Ser9 of helSyn is highlighted. (C) Schematic representation of the helSyn structure. To generate a mutant that could not be phosphorylated at site 1, Ser9 was substituted with Ala (helSynALA9).





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