QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. Silencing of the Apc6/Cdc16, Apc7 and Apc8/Cdc23 genes by RNAi results in mitotic abnormalities. Wild-type mitotic cells in prometaphase (A), metaphase (B) and anaphase (C). Neuroblast cells from both Apc6/Cdc16 (E-G) and Apc8/Cdc23 (I-L) knocked down larvae show metaphase-like arrest with overcondensed chromosomes. The chromosomes in most of the arrested cells appear scattered (E,G,I,K), in about 10% of mitotic cells the chromosomes are locked at the metaphase plate (F,J). Some degree of chromosome overcondensation could also be observed in anaphase figures (L) together with irregular chromosome segregation. Some cells appear polyploid (G,K), and they invariantly show chromosome overcondensation. Overcondensed chromosomes sometimes appear to be in the process of decondensation (G). Induction of Apc7-specific RNAi causes a mild mitotic phenotype. Neuroblast cells from Apc7^RNAi larvae show signs of uneven chromosome condensation and telomeric fusions (D) and occasionally chromosome bridges in anaphase (H).

Return to article