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Figure 5


Fig. 5. Cyclin B is not degraded in RNAi silenced Apc6/Cdc16 and Apc8/Cdc23 cells. Colours are the same as on Fig. 4. In wild-type cells (n=10) the cyclin B level is high in prophase and prometaphase (A-D) and it starts to diminish at or after the onset of metaphase (E-H). Cyclin B staining is quite pronounced in more than 80% of RNAi silenced Apc6/Cdc16 (I-L) and Apc8/Cdc23 (M-P) cells (n=20 and 17, respectively) arrested in metaphase indicating that cyclin B is not degraded.





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