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Fig. 6. Disrupting nucleocytoplasmic shuttling of constitutively activated STAT3 in v-Src-transformed fibroblasts results in apoptotic cell death. Mouse 3T3 fibroblasts and v-Src-transformed mouse fibroblasts were cultivated in six-well plates for 72 hours and then subjected to further analysis. Cells were either left untreated (–), were treated with ratjadone A for the last 24 hours (+) or treated with ratjadone for 24 hours and subsequently incubated for another 24 hours without ratjadone A (+/–). (A) Phase contrast images of the cells after 72 hours. (B) Cell lysates were subjected to SDS-PAGE and western blotting. pY⁷⁰⁵-STAT3 and STAT3 were detected by immunostaining. (C) Cells were harvested, stained with annexin V-FITC and analyzed by flow cytometry. The non-treated cell population is shown in grey in all histograms. The histograms of ratjadone-A-treated cells are shown as black lines.

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