spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Chromatin remodelling can be induced in MRC5 fibroblasts by IFN{gamma} and sodium butyrate (SB). (A) FISH image showing HLA-DRA (red) extending from the painted CT6 (green) in MRC5 cells after treatment with SB for 1 hour. (B) Comparison of external chromatin loops for the MHC and the 6p24 region in untreated control cells, in cells treated with IFN{gamma} for 24 hours and in cells treated with SB for 1 hour (n>=100). A significant increase in the percentage of external chromatin loops was observed for all three MHC regions in IFN{gamma} and SB-treated fibroblasts compared with untreated cells. (C) External chromatin loops carrying HLA-DRA induced by SB treatment over a 1-hour time course. (D) Comparison of external chromatin loops induced by IFN{gamma} over a 24-hour time course in control cells (without IFN{gamma}) and cells treated with SB for the last 1 hour of the IFN{gamma} treatment. The mean percentage of signals on external chromatin loops is represented on the y-axis, error bars show the standard deviation. The value at the 0-minute (0') time point for MRC5+SB+IFN{gamma} is high because the cells have been exposed to SB for 1 hour.





Right arrow Return to article