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Fig. 3. H2O2-induced apoptotic cells display NOS-like activity. (A) NOS activity assessed in untreated and H2O2-treated wild-type cells. The radioactivity obtained from a negative control consisting of yeast extract boiled for 20 minutes was subtracted from all the samples to remove background radioactivity. Data are expressed as the percentage conversion of L-[3H]arginine to [3H]citrulline. *P
0.03 versus untreated cells; t-test, n=4. (B) Intracellular amino acid concentrations of untreated (control) and H2O2-treated wild-type cells. *P
0.05 versus untreated cells; t-test, n=3.