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Figure 4


Fig. 4. CAP-Gly-containing domains are targeted to the Golgi-like network and CAP350 depletion induced Golgi complex scattering. Cells expressing either the Ser-rich CAP-Gly domain (984-2589) (A,B) or the C-terminus (1896-3117) (C) in fusion with a 6xMyc tag were fixed 24 hours after transfection with methanol after Triton X-100 extraction. Cells were subsequently stained with the anti-Myc antibody (green) and the Golgi antibody (red) CTR433, or A24 antibody which recognises the AKAP450 protein and stains the Golgi-like network. (A) The Ser-rich CAP-Gly domain is targeted to the centrosome when expressed at a low levels. (B) At higher expression levels, a Golgi-like network is decorated with the Myc antibody. (C) The C-terminal domain is also targeted to the Golgi-like network but no centrosome staining is observed. (D) Diagram representing the CAP350 protein with the Ser-rich regions in yellow and the CAP-Gly domain in blue. The different domains that were used for overexpression are shown below in green. The localisation of the overexpressed proteins are indicated for each construct in the table. Bar, 20 µm.





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