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Fig. 7. Microtubules are less stable in CAP350-depleted cells. Cells were transfected with GL2 (A) or CAP350 (B) duplexes for 48 hours and further treated with 5 10–6 M Nocodazole for 10 minutes and stained for CAP350 and tubulin. Note the depletion of CAP350 at the centrosome and the decrease in the microtubule number after CAP350 siRNA treatment. All images have been scaled identically for fluorescence intensity using Metamorph software to directly compare the decrease in siRNA CAP350 cells at the centrosome and in the microtubule pattern. Bar, 20 µm.