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Figure 4


Fig. 4. Transient and stable loss of TI-VAMP does not affect the establishment of a polarized monolayer on filters. FRT cells stably expressing PLAP, FRT cells stably expressing PLAP electroporated with 100 pmol of siRNA targeting rat TI-VAMP or against human beta-globin, and FRT si TI-VAMP::PLAP cells, were grown on filters for 4 days. (A) Transepithelial resistance (TER) was measured every day. Note that there is no difference in the establishment of polarity between control (siRNA beta glo) and TI-VAMP transient-knockdown cells (siRNA TI-VAMP) and between control (FRT PLAP) and TI-VAMP stable-knockdown cells (FRT si TI-VAMP::PLAP). (B) Confocal X-Y and Z sections at the level of tight junctions of control (siRNA beta glo), TI-VAMP transient-knockdown (siRNA TIVAMP) and TI-VAMP stable-knockdown cells show that the integrity of the tight junctions is preserved in each case. Bars, 10 µm.





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