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First published online September 18, 2007

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NO signals for yeast suicide

Nitric oxide (NO) has both pro- and anti-apoptotic functions in mammalian cells. Its pro-apoptotic functions are partly exerted through S-nitrosation (covalent attachment of NO to cysteine residues) of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which moves to the nucleus and triggers apoptosis. Paula Ludovico and colleagues now report that NO signalling plays a similar pro-apoptotic role in yeast, where the existence of apoptosis has been hotly debated (see p. 3279). The authors show first that yeast cells synthesize NO from L-arginine upon hydrogen-peroxide-induced apoptosis, even though they lack an orthologue of mammalian NO synthase. This NO, the authors report, induces the generation of intracellular reactive oxygen species (ROS), which can drive mitochondria-dependent apoptosis, and S-nitrosation of GAPDH. Inhibition of NO synthesis decreases S-nitrosation of GAPDH and ROS accumulation, and increases cell survival, the authors report. Finally, they show that NO and GAPDH are involved in physiological cell death during chronological aging of yeast cells. Ludovico and colleagues therefore conclude that the pro-apoptotic functions of NO are evolutionarily conserved and play a role in senescence.

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NO-mediated apoptosis in yeast

Bruno Almeida, Sabrina Buttner, Steffen Ohlmeier, Alexandra Silva, Ana Mesquita, Belém Sampaio-Marques, Nuno S. Osório, Alexander Kollau, Bernhard Mayer, Cecília Leão, João Laranjinha, Fernando Rodrigues, Frank Madeo, and Paula Ludovico
JCS 2007 120: 3279-3288. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?