JCS013698 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF
• Supplemental Table S2 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Unclustered kinetochores are associated with the inner face of the nuclear membrane. Log phase Δmto1 cut11-gfp fta2-tdtomato cells were fixed and stained with DAPI and visualised by fluorescence microscopy. An interphase cell is shown in the top row and a mitotic cell shown in the bottom row. Nuclear membrane is shown in green, kinetochores are shown in red and chromatin is shown in blue. Arrowhead indicates a region of membrane deformation during mitosis. Bar, 2 μm.

• Supplemental Figure S2 -

  Fig. S2. Pkl1 and Klp2 are not required for the retrieval of unclustered kinetochores. (A) The distance between an unclustered kinetochore pair and the spindle pole was calculated at 30-second intervals in multiple movies of Δpkl1 Δklp2 Δmto1 ndc80-gfp cdc11-cfp cells. (B) Images from a representative movie used A. The position of the unclustered kinetochores is shown by a white arrowhead. Bar, 1 μm.

• Supplemental Figure S3 -

  Fig. S3. Retrieval of unclustered kinetochores in the absence of Dam1 and Klp5. Images from a representative movie of (A) Δdam1 Δmto1 ndc80-gfp cdc11-cfp cells and (B) Δklp5 Δmto1 ndc80-gfp cdc11-cfp cells. The position of the unclustered kinetochores is shown by a white arrowhead. Bar, 1 μm.