spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Effect of the overexpression of the RBP-J{kappa} mutant on the prostanoid biosynthesis pathway. Cells were transfected with RBP-J{kappa} DN (a mutated form of RBP-J{kappa}) or empty (mock) plasmids and treated for 6, 24 or 48 hours with IL-1beta (10 ng/ml) or vehicle (control). (A) PLA2-IIA transcripts were assayed by RT-PCR. Results are expressed as a percentage of the PLA2-IIA mRNA level of control cells and represent the mean ± s.e.m. of three independent experiments. (B) Secretion of PLA2 and PGE2 was expressed as a percentage of that of control cells. The basal PLA2 secretion from mock-transfected cells was 3.6±0.9 and 4.1±0.6 pmol/min/µg RNA, 24 and 48 hours after transfection, respectively. For RBP-J{kappa} DN-transfected cells, this parameter was 4.9±0.7 and 4.5±0.8 pmol/min/µg RNA. The basal PGE2 secretion levels from mock and RBP-J{kappa}-DN-transfected cells were 16.9±1.5 and 18.3±0.9 pg/ml, respectively. The data represent the mean ± s.e.m. of four to five independent experiments. RBP-J{kappa} DN versus mock: *, P<0.05; **, P<0.01.





Right arrow Return to article