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Figure 1


Fig. 1. The hi2217 mutant exhibits a chronic inflammation phenotype and reduced expression of Hai1. (A) Genomic map of hi2217 insertion; exons are depicted as numbered boxes, and the start of the Hai1 (HAI-1) open reading frame (blue) is indicated (ATG); each section equals 500 bp. (B-F') Images of hi2217 embryos; B-G are wild-type siblings, B'-G' are mutants. (B,B') In situ hybridization of zebrafish MPO at 2 dpf, arrows indicate the position of the intermediate cell mass (ICM), the location of neutrophil development in zebrafish embryos. (C,C') Oblique coherent contrast (OCC) images of live hi2217 embryos at 26 hpf, arrows indicate clusters of rounded cells on the yolk sac (ys) and yolk sac extension (yse) of the mutant. (D-E') Hematoxylin and Eosin-stained transverse sections of hi2217 embryos at 2 dpf, at the level of the yolk sac extension (yse); s, somites; n, notochord. D,D' are full views; E,E' are magnified views of the boxed areas in D,D'. (F-G') In situ hybridization of Hai1 at 30 hpf. (F,F') Whole-mount and (G,G') frozen sections at the level of the yolk sac, which is indicated by dashed lines in F,F'; arrowheads indicate the epidermis. Whole embryos are oriented anterior to the left, dorsal side up; sections oriented dorsal side up. Bars, 200 µm.





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