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Figure 5


Fig. 5. Injection of hai1 MO into wild-type embryos phenocopies the hi2217 mutant. Shown are images of wild-type embryos at 24 hpf (A,A') and 48 hpf (B-E) following injection of a standard control MO (A), hai1 5-base mismatch MO (B,C) or hai1 MO (A'-C'). (A,A') Live OCC images; (B,B') OCC images and (C,C') corresponding zMPO immunolabeling in fixed embryos. (D,E) Partial knock-down by co-injection of hai1 MO and TMR-dextran (to trace injected cells) into 8- to 16-cell wild-type embryos. (D) OCC overlaid with red fluorescence from TMR-dextran, indicating cells that contain the MO, (E) Red fluorescence (TMR-dextran) overlaid onto green zMPO immunolabel. Note the otherwise wild-type morphology of the tailfin (compare to B,B') with localized areas of rounded cells (arrows) that overlap with red fluorescent cells, to which neutrophils (green) are recruited. Embryos are oriented anterior to the left, dorsal side up. Bars, 100 µm.





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