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Fig. 8. Neutrophils in hi2217 mutant embryos display random migration as compared to wild-type neutrophils responding to a wound, but retain the ability to respond directionally to acute injury. (A,B) `Mutant' data are derived from the first 12 minutes of each hi2217 mutant track, `WT Wound' and `Mutant Wound' data are derived from tracks of neutrophils in response to tailfin wounds in mutants and wild-type siblings, respectively. (A) Table summarizing cell tracking data, including the number of cells tracked (#), average velocity (V, µm/minute) and average directional persistence (D/T); standard deviations are listed in parentheses. (B) Plot of average velocity (µm/minute, y axis) against directionality (D/T, x axis). Each point indicates the parameters for an individual cell; blue points are homozygous mutants, red points are wild-type siblings, yellow points are wounded mutants; dashed lines indicate the average values for each data set. (C) Plot of average mean displacement (µm, y axis) against square root of time interval (minute^1/2, x axis) for the first 8.5 minute of each track; error bars indicate the standard error of the mean. Blue points are homozygous mutants, red points are derived from tracks of neutrophils in response to tailfin wounds in wild-type siblings. (D,E) Mutant neutrophil response to tissue injury. (D) A homozygous mutant hi2217;MPO:GFP embryo at 2 dpf was briefly analyzed by time-lapse microscopy: a single neutrophil (arrows indicate the same cell in each frame) is tracked to an area of cell rounding (yellow brackets) in the ventral fin; indicated times are from the start of the movie. (E) The same embryo was then wounded (indicated with *) to the left of the bracketed area, and neutrophils (arrows or arrowheads mark two individual cells in each frame) were observed to migrate to the wound, with several neutrophils at the wound by 7.5 minutes (indicated times are post-wound). Tracks for four individual neutrophils are overlaid in the last frame. Bar, 50 µm.

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