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Figure 1


Fig. 1. Nesprin-3 is displaced from the NE upon overexpression of nesprin-1 and nesprin-2. (A) NIH3T3 cells were transiently transfected with constructs encoding GFP-dnNesprin-1 or GFP-dnNepsrin-2, fixed in paraformaldehyde and stained for endogenous nesprin-3. Representative confocal images are shown. Bars, 10 µm. (B) Quantitative analysis of the effect demonstrated in (A). The average gray value of endogenous nesprin-3 at the NE was measured for individual transfected and untransfected cells. Results are shown as the mean gray value±s.e.m. (n=50). *, P<0.001.





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