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Figure 4


Fig. 4. ErbB1 dependence of electrotactic migration. (A) Inhibition of EGF receptor signalling with AG1478 abolished electrotactic response in mammary cancer cells (see supplementary material Movie 5). Cells were plated on collagen-1 coated dishes overnight, starved in serum-free {alpha}-MEM for 3 hours before the experiment, with or without 2 µM AG1478. An EF of 1 V/cm was applied for 2 hours. Red lines and blue arrowheads represent trajectories and direction of cell movement. (B) Trajectories of cells over 2 hours with the starting points positioned at the origin. x- and y-axes give distance in µm. (C) AG1478 inhibited displacement speed in EF-induced MTLn3 electrotaxis (*P<0.001 compared with EF alone), but cells in AG1478 group still moved faster than no EF control, (^P<0.01 compared with no EF control). There are no differences in trajectory speed between the two groups. (D) Anodal directedness was largely suppressed by AG1478 to the same value as no EF control *P<0.001 compared with EF alone. Results were calculated from the means of three independent experiments.





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