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Figure 1


Fig. 1. Distribution of endogenous HP1 proteins in human and mouse cell lines. (A) Staining of HeLa and C127 cells with antibodies (Ab) to HP1{alpha}, HP1beta and HP1{gamma}. Propidium iodide (PI) staining of the cells is also shown. Arrows indicate major heterochromatic foci. (B) Double staining of the same cells with anti-HP1{alpha} (red) and anti-HP1beta (green) antibodies. (C) Double immunofluorescence as in B using anti-HP1beta (green) and anti-me3K9-H3 (red) antibodies. Arrows in the enlarged images indicate lack of colocalization. (D) Detail of HP1{alpha} and HP1beta foci (green) counter-stained with PI (red) at high contrast and higher magnification. nu, nucleolus. (E) Relative fluorescence intensity per unit of nuclear surface (I/A; in arbitrary units) in antibody-stained cells. (F) Average number of HP1{alpha} and HP1beta foci (3 µm or greater in diameter) in HeLa and C127 cells as detected after morphometric analysis. Successive optical sections and `projections' from 15 cells were analyzed in each case. Bars, 5 µm. Note: In this and all subsequent figures, merged images are displayed in color, whereas individual red and green profiles are reproduced in grayscale to minimize differences in the visual perception of the red and green color.





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