QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Fate and dynamics of newly expressed HP1-GFP proteins in human and mouse cell lines. (A) Distribution of HP1-GFP proteins expressed in HeLa and C127 cells. Representative examples of the `diffuse' (D, white asterisks) and the `speckled' (SP, blue asterisks) phenotypes are shown. (B) Relative distribution of HP1-GFP and me₃K9-histone H3 (as detected by a specific antibody) in the two cell types. Only merged images are shown (HP1-GFP: green, me₃K9-H3: red). Arrows indicate sites where the two markers do not coincide. (C) Relative fluorescence intensity per unit of nuclear surface (I/A; in arbitrary units) in samples of transfected cells. Successive optical sections and `projections' from at least 15 cells were analyzed in each case. (D) Profiles of HeLa and C127 cells transfected with a chromodomain containing (CD-GFP) and a chromodomain-lacking ( ${Delta}$ CD-GFP) mutant of HP1 $beta$ . Observe that CD-GFP assembles correctly in mouse cells, but remains diffuse in human cells. (E) Distribution of HP1-GFP proteins in the same cell lines used in (A) after S phase block with hydroxyurea. Note that HP1 ${alpha}$ /HP1 $beta$ are diffuse in human cells, but continue to form large foci in mouse C127 cells. Also notice that the targeting of HP1 ${gamma}$ -GFP in the mouse system is not affected by S phase blocking. (F) FRAP data from transfected HeLa and C127 cells. Fluorescence recovery half-times (t_1/2) are in seconds. rt_1/2 is the ratio of the half-times in the two cell types; im. fr. is the immobile fraction. Bars, 5 µm.

Return to article