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Figure 6


Fig. 6. Distribution and dynamics of HP1 proteins in wild-type and mutant MEFs. (A) Localization of endogenous HP1{alpha}, HP1beta, HP1{gamma} and me3K9-histone H3 in wild-type MEFs (WT) and HP1{alpha}-HP1beta double null mutants ({alpha}–/beta–). Propidium iodide (PI) and antibody staining (Ab) are shown. (B) Western blot on WT and {alpha}–/beta– cells with the three anti-HP1 antibodies. (C) Distribution of HP1-GFP protein variants in the two cell types used above. Propidium iodide (PI) and GFP fluorescence are shown. Notice the different distribution of HP1{gamma}-GFP in WT and {alpha}–/beta– cells. (D) The average number of HP1 foci (2 µm or greater in diameter) in transfected cells. (E) FRAP data from transfected WT and {alpha}–/beta– cells. rt1/2 is the ratio of the half-times in the two cell types; im. fr. is the immobile fraction. Bar, 5 µm.





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