spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Redistribution of HP1 in peripheral blood lymphocytes (PBLs). (A) Double staining with anti-HP1{alpha} (green) and anti-HP1beta (red) antibodies. The panel shows non-treated (NT), phytohemagglutinin-treated (PHA), lipopolysaccharide-treated (LPS) and IL-2-treated (+IL-2) human PBLs. Bar, 13 µm. Note the difference in the size of the nucleus. (B) Morphometric data showing the percentage of HP1{alpha} and HP1beta foci in the nucleus of non-treated and treated cells, as indicated (dark blue bars: 1-2 µm foci; green bars: 2-3 µm foci, orange bars: 3-4 µm foci, and light blue bars: 4-5 µm foci). Optical sections and `projections' of 15 different cells were analyzed in each case. (C) Fold-difference in the abundance of the various classes of HP1 particles upon treatment in relation to controls. The results, shown in a tabular form, were derived from the data presented in B. (D) Western blots of PBLs before and after treatment with LPS or IL-2. The blots were probed for HP1{alpha}, HP1beta and histone H3, as indicated.





Right arrow Return to article