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Fig. 6. Downregulation of HP1 and HP1 during mammalian erythropoiesis. (A) Staining of HMBA-treated mouse erythroleukemia cells (MEL) with anti-HP1 or anti-HP1 antibodies (green) and propidium iodide (red). Arrows indicate differentiated, normoblast-like figures possessing condensed nuclei. Cells with bigger nuclei are undifferentiated early erythroblasts that have not responded to HMBA treatment (for more details see text). Bar, 5 µm. (B) The same experiment as in A using human erythroleukemic cells (K-562). Bar, 5 µm. (C) Immunohistochemical detection of HP1 and HP1 in human bone marrow sections. The images show erythropoietic islands containing early and late erythroblasts (red arrows) stained with the corresponding antibodies and Hematoxylin. (D) Double staining of undifferentiated and partially differentiated mouse eythroleukemia cells (as in A) with anti-HP1 (green) and anti-histone modification (red) antibodies. Note that the anti-me₃K9 and anti-me₃K27-H3 antibodies readily penetrate the condensed nuclei of the normoblasts, indicating access to intranuclear antigens. Bar, 5 µm.

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