QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Three RCC1 transcript variants are expressed in humans. (A) Linear representation of RCC1 protein domains (not to scale), showing sequence alignment of the NTR (residues 1-27) of human RCC1 protein with insert-containing RCC1 isoforms from: human (hs); chimpanzee (pt); rhesus monkey (macm); golden hamster (ma); mouse (mm); and African clawed frog (xl). RCC1 homologues in species other than human are not shown. Insert sequences are shown in bold. Phosphorylation sites (serines 2 and 11) are indicated by circles labelled P. (B) Schematic showing how alternative mRNA splicing around and within exon 6' could generate the known RCC1 transcript variants. (C) Isoform-specific antibodies were generated to peptides corresponding to RCC1 sequences. Anti-R-INS should detect insert-containing isoforms, whereas the anti-R-DIS epitope should be disrupted by them. (D) Precipitation of RCC1 proteins from asynchronous HeLa cell extracts by GST-RanT24N or GST as a control, with analysis by immunoblotting with RCC1 isoform-specific antibodies. (E) Immunoblotting of human cultured cell lysates. (F) Immunoblotting of normal human tissue lysates on an Instablot (IMGENEX) membrane (Cambridge Bioscience), which is pre-loaded with SDS-PAGE-separated normal tissue lysates (20 µg protein/lane). The blot was probed using anti-R-INS (upper panel), then re-probed using anti-RCC1 (lower panel). RCC1 isoforms are indicated, with the question mark signifying a possible RCC1 band of unknown identity. Molecular mass (in kDa) is indicated on the left of the immunoblots.

Return to article