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Fig. 4. RCC1 ${gamma}$ interacts less well with importins than RCC1 ${alpha}$ . (A) Endogenous importin $beta$ precipitated from asynchronous HeLa extracts using beads coupled to GST-RCC1 ${alpha}$ , GST-RCC1 ${gamma}$ or GST, with or without His₆-importin ${alpha}$ 3 (Imp ${alpha}$ 3). (B) Endogenous RCC1 precipitated from asynchronous HeLa extracts using beads coupled to GST-importin $beta$ (Imp $beta$ ) or GST, with or without His₆-importin ${alpha}$ 3. (C) Binding of untagged RCC1 ${alpha}$ , RCC1 ${gamma}$ or GST to the chromatin pellet from subcellular fractionation of HeLa cells, with His₆-importin ${alpha}$ 3 and GST-importin $beta$ , or with GST as a control. (D) Endogenous RCC1 isoforms from asynchronous HeLa extracts bound by DNA-cellulose, with or without His₆-importin ${alpha}$ 3 and GST-importin $beta$ . Molecular mass (in kDa) is indicated on the left.

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