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First published online September 19, 2007

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Controlling the Rabl

We know a lot about the mechanisms that control capture of chromosomes by spindle microtubules at mitosis in budding yeast. But how this occurs in fission yeast and what ensures sister chromatids attach to microtubules emanating from opposite poles (`bi-orientation)' are less clear. On p. 3345, Jonathan Millar and colleagues reveal the part the ten-component Dam1/DASH complex plays by examining its role in fission yeast that have a disrupted `Rabl' configuration. During interphase, centromeres are clustered at the nuclear envelope in a region closest to the spindle pole body (this is the so-called Rabl configuration). In cells that lack the centrosomin-like protein Mto1, the Rabl configuration does not form correctly – one pair of sister chromatids becomes unclustered from the others. The researchers used 3D live-cell fluorescence microscopy to examine what happens to these as the cells enter mitosis. Analysing Dam1 mutants, they find that the Dam1/DASH complex is needed for intranuclear spindle microtubules to retrieve unclustered kinetochores (the complexes that link sister chromatids to microtubules). When they watch this process in more detail, they see that (unlike in budding yeast) unclustered kinetochores are retrieved at the plus end of a depolymerising spindle microtubule. The authors suggest that the Dam1/DASH complex couples the movement of kinetochores to depolymerisation of spindle microtubules.

Related articles in JCS:

The Dam1/DASH complex is required for the retrieval of unclustered kinetochores in fission yeast

Alejandro Franco, John C. Meadows, and Jonathan B. A. Millar
JCS 2007 120: 3345-3351. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content