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Figure 2


Fig. 2. The SYT1 C2B domain inhibits migration and invasion by TAM2D2 cells. (A) FACS analysis of GFP in the C2B transfectants. Filled areas represent the fluorescence in the transfectants; open areas, the fluorescence in the untransfected control cells. C2B(h), cells sorted for high GFP; C2B(m), sorted for medium GFP levels. (B) Migration towards 100 ng/ml CXCL12. Results are given as the mean ± s.e.m. of the migration index, which is set to 1 for the control cells in each individual experiment. Shown are averages of 14 experiments. (C) Invasion into REF monolayers. Results are given as the mean ± s.e.m. of the invasion index, which is set to 1 for the control cells in each individual experiment. Shown are averages of eight experiments. In the absence of CXCL12, no migration of TAM2D2 cells was observed at all.





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