spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Synaptotagmin 3 downregulation by antisense transfection impairs migration. Western blot analysis of SYT3 protein levels in Syt3 transfectants (S, TAM2D2-Syt3), antisense Syt3 transfectants (AS, TAM2D2-anti-sense SYT3), and untransfected cells (T, TAM2D2). After SYT3 staining, blots were stripped and re-probed with actin antibody. Migration assay shows migration towards 100 ng/ml CXCL12, as in Fig. 2B. Data are averages of three experiments. Shown are results shortly after transfection (i.e. after sorting of GFP-high cells and growing of sufficient cells) and 1 month later, in two separate experiments (A and B). Experiments were done with separate sets of cells, transfected on two different occasions.





Right arrow Return to article