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Figure 6


Fig. 6. Synaptotagmin 3 localization in TAM2D2 cells. (A) TAM2D2-SYT3-GFP transfectants and (B) TAM2D2 cells were stained with SYT3 antibody and observed using confocal microscopy. Three cells are shown. (C) TAM2D2-SYT3-GFP transfectants were stained with GFP antibody bound to the gold particles and observed with the electron microscope. (Right panel) Magnified view of a multi-vesicular body. (D) Number of gold particles per µm2 in MVBs, cytosol, mitochondria, perimembrane area and Golgi apparatus. Images of 25 cells were examined.





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