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Fig. 6. Effect of FPs on cardiomyocyte beating activity. (A) SMA-FP and SKA-FP (50 µg/ml) were applied on differentiated EBs at day 8 for 2 hours. (B) The frequency of beating was estimated by counting under the microscope the number of beats per 30 seconds of about 50 EBs during a 2-hours period without any treatment (
) or following a treatment with SMA-FP (
) or SKA-FP (
). (C) 20-second movies of beating EBs were recorded and the variation of grey level in regions at the periphery of EB was calculated over time. Plots are representative of the EBs beating activity before (a and c) and after 2 hours treatment with 50 µg/ml of either SMA-FP (b) or SKA-FP (d). (D) To obtain beating rate spectrums of the frequency plots, Fourier transformation was performed on plots shown in C (see Materials and Methods for details). Numbers on the y-axis represent an `index of arrhythmia'.
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