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Figure 1


Fig. 1. Insulin-positive cells in the hilar region. (A) Bile duct system of an adult mouse made visible by injection of the gall bladder with 0.5% Trypan Blue. The boxed area is the hilar region where the ectopic cells are located. gb, gall bladder; panc, pancreas; duo, duodenum. (B) Whole-mount stain of the ducts in the hilar region of an adult mouse using Dolichos biflora lectin (red), and antibody against insulin (green). The insulin-positive cells are in clusters in the connective tissue surrounding the ducts. Bar, 500 µm. (C-F) Insulin-positive cells (green), associated with bile ducts: (C) 18.5-day embryo, (D) 1 day postnatal, (E) 7 days postnatal, (F) 6 months. (G) Two insulin-positive cells just within the liver parenchyma of a 3-week-old mouse. Bars, 20 µm. (H) Analysis of the hilar duct system in 30 mice of different ages (E18.5 to age 6 months). Each vertical line represents a series of serial sections from one mouse of which every fifth slide (five sections) was examined. The horizontal bars indicate those sections containing one or more insulin-positive cells.





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