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Figure 1


Fig. 1. Induction of Cdc2 protein in regenerating sciatic nerves. (A) Western analysis showed a strong but transient increase of Cdc2 protein in 2 cm nerve segments proximal and distal to the injury site. (B) Distribution of Cdc2 protein around the injury site. Vertical arrow indicates the injury site. (C) RT-PCR for Cdc2 mRNA expression in 1 cm nerve segment proximal (P) or distal (D) to the injury site. (D) FISH analysis of Cdc2 mRNA in the sciatic nerve. Sciatic nerve sections were used for FISH with antisense or sense riboprobe and immunofluorescence staining with anti-S100beta antibody. (E) Immunofluorescence views show that signals for S100beta and Cdc2 proteins are colocalized in the nerve sections. In D and E, transverse nerve sections (20 µm) 0.5 cm distal to the injury site were prepared at 7 d.p.c. Actin was detected in A-C as internal loading controls. Bars, 50 µm.





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