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Fig. 2. Cdc2 expression in cultured Schwann cells. (A) Western blot analysis of Cdc2 in cultured Schwann cells. Schwann cells were prepared from sciatic nerves given crush injury for 0-14 days or from the sciatic nerve of the rat of postnatal day 3 (pnd3). Cells were incubated for 2 days in 10% serum and 2 µM forskolin (FSK) (+) or 0.5% serum and 0 µM forskolin (–). Cdc2 protein in Schwann cells was increased by injury preconditioning of the sciatic nerve and further upregulated by serum and forskolin treatment. Actin was detected as an internal loading control. (B) Merged views for the localization of S100
- or Cdc2-positive cells in the total cell population (Hoechst 33258 stained) or Cdc2-positive cells in S100-positive cell population. Schwann cells were prepared from sciatic nerves with or without injury preconditioning for 7 days. Cells positive to S100 and/or Cdc2 were increased by injury preconditioning, and forskolin and serum treatment. Bar, 20 µm.
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