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Fig. 3. Cdc2 activity is required for Schwann cell migration. (A) Immunofluorescence staining of explant culture for the detection of S100
-positive Schwann cells (red) and Cdc2-positive cells (green). Enlarged image of the rectangle is shown in the inset. The merged image indicates that most migrating Schwann cells in injury-preconditioned explants express Cdc2. Quantification data of Schwann cell migration in the explant culture with or without injury preconditioning (Injury-pre. vs. Non-pre) are shown on the right. The arrow indicates the direction of migrating Schwann cells from the sciatic nerve center. (B) Comparison of Schwann cell migration in culture between injury-preconditioned and non-preconditioned groups. The data represent Schwann cell migration as percentage of total cells on the filter culture recovered from injury-preconditioned or non-preconditioned sciatic nerves. (C) Dose-dependent decrease of Schwann cell migration by roscovitine treatment. Injury-preconditioned Schwann cells were cultured under the different concentrations of roscovitine for 2 days. Statistical comparisons were made compared with the cell group treated with 0 µM roscovitine (*P<0.05; ***P<0.001). In B,C, migrated Schwann cells on the coverslip were immunostained with anti-S100 antibody, and the cells on the top of the filter were stained with Cresyl Violet. The cells from ten random fields were counted. (n=3 for A and n=4 for B,C). Bars, 50 µm (A); 20 µm (inset in A).
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