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Figure 3


Fig. 3. Yap8p stabilization contributes to increased transcriptional activity. An ACR3-promoter-lacZ fusion construct was transformed into wild-type cells and into ubiquitin-proteasome pathway mutants. Transformants were assayed for beta-galactosidase activity as described in Materials and Methods. The results are the average of three independent experiments and the error bars represent s.d. (A) beta-galactosidase activity measurements in the pre1-1 pre4-1 mutant (WCG4a background). (B) beta-galactosidase activity measurements in ubc4{Delta} and ubc5{Delta} mutants (DF5 background). (C) beta-galactosidase activity measurements in wild-type cells (WCG4a background) transformed with an empty vector or with a plasmid carrying Yap8p-HA.





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