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Fig. 7. The fusion of NoLS to MafG makes it possible to delocalize the nuclear complex MafG-P45 to nucleoli. HeLa cells were cotransfected with GFP-P45 and DsRed-B23 (a-d), with DsRed-MafG and GFP-B23 (e-h), with DsRed-NoLS-MafG and GFP-B23 (i-l), with DsRed-NoLS-MafG and GFP-P45 (m-p), with DsRed-NoLS and GFP-P45 (q-t) or with DsRed-NoLS-MafG and GFP-sirtuin-1 (u-x). P45 (a) and MafG (e) were localized in the nucleus and excluded from the nucleoli as observed by comparing with B23 localization (compare a,b and e,f) and by the superimpositions of labelings (c,g). When fused to NoLS, MafG (i) colocalized with B23 (j) in nucleoli as evidenced by the superimposition of both labelings (k). Targeting of MafG to nucleoli by addition of the NoLS induced a clear relocalization of P45 to the nucleoli (compare m,n,o and the phase contrast image, p). Coexpression of DsRed-NoLS and GFP-P45 (q-t) showed that the expression of NoLS did not modify the nuclear localization of P45. By contrast to P45, targeting of MafG to nucleoli by the addition of NoLS (u) did not modify the nuclear localization of sirtuin-1 (v). Bar, 10 µm.
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