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Figure 6


Fig. 6. Rac1 and Cdc42 are essential for the increased F-actin polymerization induced by Abeta1-42 treatment. Primary hippocampal cells treated with Abeta1-42 were transfected with the dominant negative forms of RacT17N-GFP and Cdc42T17N-GFP (arrows), and then stained for F-actin using Rhodamine-phalloidin. The untransfected neurons are indicated by arrowheads. Scale bar, 20 µm. (B) Changes in F-actin levels were determined and differences between transfected (t) vs. untransfected (ut) control and Abeta-stimulated neurons were determined using Student's t-test. The values were expressed as mean ± s.e.m. for three different experiments, with at least 12 determinations per experiment; *P<0.05.





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