spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Soluble ephrins destabilise neural crest cell lamellipodia. (A) Neural crest cells were examined using timelapse videomicroscopy for 30 minutes and then stimulated with 2 µg/ml pre-clustered ephrin-B2-Fc for 45-60 minutes. The withdrawal of neural crest cell lamellipodia (red arrow) was associated with membrane ruffling (red arrowhead) following contact with the ephrin-B2-Fc stripe. (B) Membrane ruffling led to the internalisation of phase bright vesicles. (C) Lamellipodial dynamics were analysed by kymography prior to stimulation (15.5±1.2 peaks/hour) and in the presence of 2 µg/ml pre-clustered ephrin-B2-Fc (25.2±1.1 peaks/hour); in the presence of ephrin-B2-Fc the lifetime of protrusions was decreased (P<0.05, n=9). A representative kymograph from a total of nine neural crest cells examined is shown (three separate timelapse movies). (D) A trace of the kymograph shown in C. Bars, 10 µm.





Right arrow Return to article