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Figure 8


Fig. 8. SR and SR-related splicing regulators are present in parallel but SAFB-independent nuclear protein complexes. (A,B). Sucrose gradient fractionation was as described in Fig. 5, again without treatment (A) and after treatment with micrococcal nuclease (B). The migration of individual proteins was monitored by immunoblotting. The mobility of size markers on the gradients is shown. (C,D) Under identical conditions to Fig. 5, no co-immunoprecipitation was observed (C) between SAFB2 and Tra2beta, while (D) antibodies to Tra2beta efficiently immunoprecipitated Sam68. (E-H) No co-immunoprecipitation was observed between SAFB proteins and (E) hnRNP A1; (F) SR proteins identified with the pan-SR family monoclonal antibody 10H3, (G) ASF/SF2 or (H) 9G8; (I) PTB.





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